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Coronaviruses : Methods and Protocols

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This volume aims to describe a variety of techniques that reflects the wide range of research currently performed in the field of coronavirology, and begins with an overview of current understandings of coronavirus replication and pathogenesis to introduce specialists and non-specialists to the field. The rest of the book is divided into several sections of chapters beginning with those that describe identification, diagnosis, and study of the evolution of coronaviruses. The next few chapters discuss the preparation of cells and organ cultures useful in propagating coronaviruses and titration techniques, as well as techniques for analyzing virus functions that require purification of the viruses. The next chapters describe two commonly used reverse genetics techniques for coronaviruses, and techniques detailing identification of cellular receptors, binding profiles of viral attachment proteins, and virus-cell fusion. The final chapters cover a broad spectrum of techniques to identify virus-host protein-protein interactions, confirm the functional role of these proteins in virus replication, study host cell responses through genome-wide or pathway-specific approaches, and visualize virus replication complexes. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

Authoritative and practical, Coronaviruses: Methods and Protocols appeals to a wide variety of scientists because it highlights techniques that are currently used in the coronovirology field, while also discussing practices applicable to other virology fields.

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Detail Information

Series Title

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Call Number

579.256 COR c

Publisher

New York : Humana New York., 2015

Collation

xi, 285p. : ill.

Language

English

ISBN/ISSN

9781493924387

Classification

579.256

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Life sciences
Coronaviruses
Microbiology
Medical virology
Virology

Specific Detail Info

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Statement of Responsibility

Edited by Helena Jane Maier, Erica Bickerton, Paul Britton

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